Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA
نویسندگان
چکیده
منابع مشابه
Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA.
A simple and fast RNA gel blot procedure is described that uses 50 mM NaOH to simultaneously transfer and fix RNA to a positively charged nylon membrane. The RNA is transferred in a downward direction, and transfer is routinely completed within 2.5 h. The resulting blots give increased sensitivity over existing methods without affecting RNA integrity and can be used in both radioactive and nonr...
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A rapid and easy procedure for preparing Northern blots is described. RNA is denatured with glyoxal in the presence of ethidium bromide and glycerol, then electrophoresed through agarose in a buffer that does not require recirculation. Without any additional washes, the RNA is vacuum-blotted to a nylon membrane in NaOH, which simultaneously removes the glyoxal adducts. All of these steps plus p...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1998
ISSN: 0736-6205,1940-9818
DOI: 10.2144/98253st03